Part:BBa_K4759004
BM3
a gene coding for Reducatase domain from P450BM3(the selfsufficient P450 enzyme). it has an excellent electron transfer system and the fastest substrate oxidation rate among the P450s owing to the proximity and specific positioning of its domains . Researcher has found that fusing P450s to reductase genes can improve the biocatalysis, We use this part to construct electron transfer pathway to Olep.
Usage and Biology
The P450 enzymes are redox-dependent proteins that source electrons from reducing cofactors to drive their activities. In bacterial systems, the electrostatic interactions between the ferredoxin (Fdx), P450 heme, and the reductase domains, as well as the negatively and positively charged amino acids on the Fdx iron-sulfur cluster and P450 proximal site, mediate the conformational changes of the Fdx for electron transfer to P450s. In addition, the substrate binding to P450 induces P450 conformational change to increase its preference for Fdx through electrostatic and steric complementarity. Optimizing protein-to-protein interactions using different methods to improve the electron transfer efficiency of the P450 system, known as "redox chaperone engineering", is one of the important means of engineering P450s, and fruitful progress has been made. Several studies have confirmed that the combined expression of P450, an enzyme with different RPs, can reconstruct and promote reactivity. This strategy has been widely used in the bacterial class I P450 system.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1339
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 385
Illegal BsaI.rc site found at 1309
Illegal SapI.rc site found at 865
Illegal SapI.rc site found at 1465
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